Vrije Universiteit Brussel

 

Publications on Multiple Myeloma

Laminin-1-induced migration of multiple myeloma cells involves the high-affinity 67 kD laminin receptor
  • Laminin-1-induced migration of multiple myeloma cells involves the high-affinity 67 kD laminin receptor.

    Vande Broek, I, Vanderkerken, K, De Greef, C, Asosingh, K, Straetmans, N, Van Camp, B, Van Riet, I.

    Br J Cancer 85 (9), 1387-95, 2001.

    Abstract:
    The 67 kD laminin receptor (67LR) binds laminin-1 (LN), major component of the basement membrane, with high affinity. In this study, we demonstrated that human multiple myeloma cell lines (HMCL) and murine 5T2MM cells express 67LR CD38(bright+) plasma cells in fresh multiple myeloma (MM) bone marrow (BM) samples showed weaker 67LR expression, but expression increased after direct exposure to a BM endothelial cell line (4LHBMEC). LN stimulated the in vitro migration of 3 HMCL (MM5.1, U266 and MMS1), primary MM cells and the murine 5T2MM cells. 67LR has been shown to mediate the actions of LN through binding to CDPGYIGSR, a 9 amino acid sequence from the B1 chain of LN MM cell migration was partially blocked by peptide 11, a synthetic nonapeptide derived from this amino sequence and also by a blocking antiserum against 67LR Co-injection of peptide 11 with 5T2MM cells in a murine in vivo model of MM resulted in a decreased homing of 5T2MM cells to the BM compartment. In conclusion, LN acts as a chemoattractant for MM cells by interaction with 67LR This interaction might be important during extravasation of circulating MM cells.

 

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